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( C) Representative mixed colony with both GFP+ and RFP+ fibroblasts demonstrating GFP+ perinuclear granules in red cells (white arrowheads), indicating upregulation of adiponectin expression. ( B) Quantification of GFP and RFP expression in fibroblastic stromal cells within each culture dish (n = 3 independent mice, 166 total colonies counted). In addition, spontaneous adipogenesis was assessed based on immunostaining for perilipin 1 (PLIN1, pink). Endogenous fluorescence was then amplified by immunostaining for green fluorescent protein (GFP) and red fluorescent protein (RFP). Continue to develop approach as better technology emerges (e.g.( A) Bone marrow stromal cells from 16 week old male Adipoq Cre+/mTmG+ mice were cultured at low density for 14 days to promote formation of colony-forming units (CFUs).On track to have first 7 Cre lines made by 9/2010 and characterized by 12/2010.Future enhancer cloning will be very simple and efficient.2 enhancer elements are ready for targeting into JM8A3 ES cells (this month).2 BAC Cres (Tbx22 and p63) are cloned, Krt6 is nearly done (unique challenges).All basic cassettes (CreIRESmCherry, ROSA targeting vector, etc) are complete.Initial plan is to make four BAC transgenics (Krt6-Cre, Tbx22-CreERT2, dNp63-CreERT2, Lhx7/8-Cre) and three enhancer (Visel project) CreERT2 lines (R26 knock-in).Goal: To generate 15 Cre driver strains for orofacialclefting research.new ENU induced mutant strain (on FVB.A) įaceBaseCre Driver Project:Current progress and future plans.Bambi, Osr1, Osr2, Jag2, Snai1 and Snai2, Gad1, Slc32a1, Tgfbr2, Wnt9b.new knockout strains - most on B6 129 background.

most are public, some still private, some imported and under development for distribution.

~ 50 strains in the JAX FaceBase Repository.

The Jackson LaboratoryBar Harbor, MEįaceBase Mouse Repository at JAX importation, cryopreservation, GQC, distribution Genetic tools and resources for orofacialclefting research PIs: Leah Rae Donahue, Ph.D.

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